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The
annexins are a great family of protein with more than 159 unique members well
known in a varied range of eukaryotic containing in protists, fungi, and plants
(Gerke and Stephen, 2002). Annexin (Ca2 + or phospholipid binding proteins) are circulated
all tissues during development and plant growth. Annexin has five major groups
(A-E) (Morgans and Fernandes, 1997). The
plant annexin is D group. Hofmann
et al. (2000) identified the configuration
of a plant annexin from
Capsicum annuum (annexin D11). Mos and Morgan (2004) found nine annexin
in Oryza sativa and Cancero et al. (2006) found eight
annexin in Arabidopsis thaliana. Many annexins from other plant
species have been reported to date, although an annexins systematic mapping in greatest
plants is still missing. The annexins criterion is conserved fold that detected
in plant annexins. It consists of a four-fold repeat (I–IV) of a 70 amino acid
sequence that constitutes the C-terminal (core) domain (Postupolska et al.,
2011). Annexins participate in a number of physiological processes, such as
exocytosis, cell elongation, nodule formation in legumes, maturation and stress
response. Plant has developed variouse
mechanisms to defend themselves against these fungi, which include the
production of low-molecular-weight secondary metabolites and proteins and
peptides with antifungal activity (Borad and Sriram, 2008). Wang et al (2009)
found annexin from Cynanchun komarovii seeds are involved
in osmotic stress and resistance pathogen in Arabidopsis.

On
the other hand, Alstonia scholaris is
a rubber tree that rich alkaloid. A.
scholaris used for traditional medicine not only in China but also in
Malaysia, Indonesia, Australia, Thailand, Philippines, India, and Africa (Changwichit
et al., 2011; Khyade et al., 2014; Meena et al., 2014; Mashudi, 2017).  It produced the milky latex. A. scholaris milky latex commonly used
as a general tonic, antipyretic, emmenagogue, anticholeric, aphrodisiac,
antidysentery, and vulnerary agents (Khyade et al., 2014), to remedy of ulcer,
neuralgia, remedy toothache (Pratap et al., 2013), anticancer (Baliga, 2010). Latex of A. scholaris is
gotten from A. scholaris tree in
National Pingtung University of Science and Technology, Taiwan. The plant latex
had been used for since long ago. It is major source for medical
appliance, vehicle tires, shoes, and cosmetics (Rubber Research Institute of
Thailand, 2010). Latex has many special things that
make it most vital polymers that having a lot of proteins, serum,
organelles, non-rubber particles, and
mainly
rubber particles (d’Auzac and Jacob, 1989). Rubber
particles are globular particles in which the hydro phobic rubber polymers are surrounded
by a monolayer membrane containing various kinds of lipids, proteins, and other
components (Wood and
Cornish, 2000; singh et al., 2003).

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Previous
overviews on plant annexins have summarised the current knowledge protein
expression in plants (Hofman, 2004; Mortimer et al., 2008; Laohavisit and
Davies 2009;2011) Althought, The information of  A.
scholaris latex proteins are so limited. The latex proteins there for the
investigation and identification of its proteins in rubber particles and their
transferase activity need to observe. This study works on purification of A. scholaris rubber protein latex, known
the soluble buffer for protein purification, and identified the micromorphology of lipid and rubber particle from its latex
of their size.

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